Journal: Science advances
Article Title: Peptide-guided lipid nanoparticles deliver mRNA to the neural retina of rodents and nonhuman primates.
doi: 10.1126/sciadv.add4623
Figure Lengend Snippet: Fig. 7. MH42-conjugated LNPs mediate expression in the neural retina after subretinal administration in the NHP. (A) Wide-field fundus autofluorescence imaging 48 hours after subretinal delivery of MH42 LNPs (n = 1). Circle demarks the location of the bleb, and yellow line indicates cross section that corresponds to immunohis- tochemistry. (B) Montaged ×10 confocal images of primate retinal cross sections labeled with anti-GFP antibody (red) and DAPI (blue). A montaged ×10 H&E image shows the retinal morphology in areas of GFP expression. (C to F) Confocal images (×40) of retinal cross sections costained with anti-GFP and cell-specific antibodies cone arrestin (cones), rod arrestin (rods and s-cones), RPE65 (RPE), and glutamine synthetase (Müller glia). Arrowheads represent co-localization. (C to G) Confocal cross sections (×20) labeled with CD3 (T cells; green) and IBA-1 (microglia; red) to observe the elicited immune response. GltS, glutamine synthetase.Scale bars represent 100μM.
Article Snippet: Slides were stain with antibodies specific for visual arrestin (rod and cone PRs; catalog no. sc-166383, Santa Cruz Biotechnology, Dallas, TX), IBA-1 (microglia; catalog no. 019-19741, Wako Chemicals, Richmond, VA), and CD3 (T cells; catalog no. sc20047, Santa Cruz Biotechnology, Dallas, TX).
Techniques: Expressing, Imaging, Labeling